Single-particle reconstruction of biological macromolecules in electron microscopy – 30 years
نویسندگان
چکیده
منابع مشابه
Single-particle imaging of macromolecules by cryo-electron microscopy.
Cryo-electron microscopy (cryo-EM) of biological molecules in single-particle (i.e., unordered, nonaggregated) form is a new approach to the study of molecular assemblies, which are often too large and flexible to be amenable to X-ray crystallography. New insights into biological function on the molecular level are expected from cryo-EM applied to the study of such complexes "trapped" at differ...
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The Transmission Electron Microscope provides two-dimensional (2D) images of the specimens under study. However, the architecture of these specimens is defined in a three-dimensional (3D) coordinate space, in volumetric terms, making the direct microscope output somehow "short" in terms of dimensionality. This situation has prompted the development of methods to quantitatively estimate 3D volum...
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ژورنال
عنوان ژورنال: Quarterly Reviews of Biophysics
سال: 2009
ISSN: 0033-5835,1469-8994
DOI: 10.1017/s0033583509990059